Fig.2 shows the relationship between retention time (also called elution volume) and molecular weight for SEC. The calibration curve shows this relationship graphically. In SEC, as shown in Fig.1, the elution order from the column is faster for larger molecules and slower for smaller molecules. SEC is a separation mode that sieves molecules according to their size. As a result, the elution order from the column is faster for larger molecules and slower for smaller molecules. 1, right), while molecules of large size flow out of the column without penetrating the pores (Fig. Regarding SEC, as molecules of various sizes flow through the column, molecules of small size flow slowly while penetrating deep into the pores in the packing material (Fig.
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